The play begins with three pages disputing over the black cloak usually worn by the actor who delivers the prologue. They draw lots for the cloak, and one of the losers, Anaides, starts telling the audience what happens in the play to come; the others try to suppress him, interrupting him and putting their hands over his mouth. Soon they are fighting over the cloak and criticizing the author and the spectators as well.
In the play proper, the goddess Diana, also called Cynthia, has ordained a "solemn revels" in the valley of Gargaphie in Greece. The gods Cupid and Mercury appear, and they too start to argue. Mercury has awakened Echo, who weeps for Narcissus, and states that a drink from Narcissus's spring causes the drinkers to "Grow dotingly enamored of themselves." The courtiers and ladies assembled for the Cynthia's revels all drink from the spring.