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Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing (Methods in Molecular Biology #255)
by Shaying Zhao and Marvin StodolskyFor both volumes: Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
Bacterial Artificial Chromosomes: Volume 2: Functional Studies (Methods in Molecular Biology #256)
by Shaying Zhao and Marvin StodolskySeveral developmental and historical threads are woven and displayed in these two volumes of Bacterial Artificial Chromosomes, the first on Library Construction, Physical Mapping, and Sequencing, and the second on Fu- tional Studies. The use of large-insert clone libraries is the unifying feature, with many diverse contributions. The editors have had quite distinct roles. Shaying Zhao has managed several BAC end-sequencing projects. Marvin Stodolsky during 1970–1980 contributed to the elucidation of the natural b- teriophage/prophage P1 vector system. Later, he became a member of the Genome Task Group of the Department of Energy (DOE), through which s- port flowed for most clone library resources of the Human Genome Program (HGP). Some important historical contributions are not represented in this volume. This preface in part serves to mention these contributions and also briefly surveys historical developments. Leon Rosner (deceased) contributed substantially in developing a PAC library for drosophila that utilized a PI virion-based encapsidation and tra- fection process. This library served prominently in the Drosophila Genome Project collaboration. PACs proved easy to purify so that they substantially replaced the YACs used earlier. Much of the early automation for massive clone picking and processing was developed at the collaborating Lawrence Berkeley National Laboratory. However, the P1 virion encapsidation system itself was too fastidious, and P1 virion-based methods did not gain popularity in other genome projects.
Bacterial Biofilms (Current Topics in Microbiology and Immunology #322)
by Tony RomeoThroughout the biological world, bacteria thrive predominantly in surface-attached, matrix-enclosed, multicellular communities or biofilms, as opposed to isolated planktonic cells. This choice of lifestyle is not trivial, as it involves major shifts in the use of genetic information and cellular energy, and has profound consequences for bacterial physiology and survival. Growth within a biofilm can thwart immune function and antibiotic therapy and thereby complicate the treatment of infectious diseases, especially chronic and foreign device-associated infections. Modern studies of many important biofilms have advanced well beyond the descriptive stage, and have begun to provide molecular details of the structural, biochemical, and genetic processes that drive biofilm formation and its dispersion. There is much diversity in the details of biofilm development among various species, but there are also commonalities. In most species, environmental and nutritional conditions greatly influence biofilm development. Similar kinds of adhesive molecules often promote biofilm formation in diverse species. Signaling and regulatory processes that drive biofilm development are often conserved, especially among related bacteria. Knowledge of such processes holds great promise for efforts to control biofilm growth and combat biofilm-associated infections. This volume focuses on the biology of biofilms that affect human disease, although it is by no means comprehensive. It opens with chapters that provide the reader with current perspectives on biofilm development, physiology, environmental, and regulatory effects, the role of quorum sensing, and resistance/phenotypic persistence to antimicrobial agents during biofilm growth.
Bacterial Biopolymers
by Bolin Kumar KonwarBacterial biopolymers are a competitive new area of research with potential applications in agriculture, petroleum exploration, and use as biodegradable plastics. This new volume provides comprehensive coverage of bacterial biopolymers. The book elucidates the main classes of bacterial biopolymers—polysaccharides and polyesters or polyhydroxyalkanoates (PHAs)—along with their definition, classification, detection, extraction, characterization, fermentation process, structure and properties, applications and uses, functions, and more. The volume covers Detection, extraction, and characterization of bacterial biopolymers Molecular identification of bacterial isolates The fermentation process Structure and properties of PHAs Biochemical characterization of the PHA-producing bacterial strains In vitro biodegradation study of PHA film by soil microorganisms PHAs for enhancing the stability of colloidal silver nanoparticles (SNP) This volume is an important source of information on the concerned bacteria, the biochemical characteristics of biopolymers, and their potential use.
Bacterial Biopolymers
by Bolin Kumar KonwarBacterial biopolymers are a competitive new area of research with potential applications in agriculture, petroleum exploration, and use as biodegradable plastics. This new volume provides comprehensive coverage of bacterial biopolymers. The book elucidates the main classes of bacterial biopolymers—polysaccharides and polyesters or polyhydroxyalkanoates (PHAs)—along with their definition, classification, detection, extraction, characterization, fermentation process, structure and properties, applications and uses, functions, and more. The volume covers Detection, extraction, and characterization of bacterial biopolymers Molecular identification of bacterial isolates The fermentation process Structure and properties of PHAs Biochemical characterization of the PHA-producing bacterial strains In vitro biodegradation study of PHA film by soil microorganisms PHAs for enhancing the stability of colloidal silver nanoparticles (SNP) This volume is an important source of information on the concerned bacteria, the biochemical characteristics of biopolymers, and their potential use.
Bacterial Biosurfactants: Isolation, Purification, Characterization, and Industrial Applications
by Bolin Kumar KonwarThis new volume offers comprehensive coverage of bacterial biosurfactants, the competitive new area of research that has exciting potential application in agriculture and petroleum exploration. The book helps readers to understand the synthesis of biosurfactants by some specific bacteria, their culture, and extraction toward use in bioremediation and enhanced crude oil recovery. The volume covers the gamut of topics in bacterial biosurfactants in nanostructure, including their comparison to synthetic surfactants, their interaction with microorganisms, and their biochemistry, characterization, genetics of production, bioremedial effects, and more. The volume also explores the myriad uses of bacterial biosurfactants, including in laundry detergents, cosmetics, food production, petroleum, agriculture, medicine and therapeutics, environment, metallurgy, etc. Attention to biosurfactants has been gradually increasing in recent years due to the possibility of their production through fermentation technology and their potential applications in environmental protection. Despite their numerous advantages over synthetic chemical surfactants, biosurfactants have been unable to compete with chemically synthesized surfactants due to high production costs in relation to the inefficient bioprocessing techniques, poor strain productivity, and use of costly substrates. This volume helps to identify the factors that need to be addressed to reduce the cost of production of biosurfactants.
Bacterial Biosurfactants: Isolation, Purification, Characterization, and Industrial Applications
by Bolin Kumar KonwarThis new volume offers comprehensive coverage of bacterial biosurfactants, the competitive new area of research that has exciting potential application in agriculture and petroleum exploration. The book helps readers to understand the synthesis of biosurfactants by some specific bacteria, their culture, and extraction toward use in bioremediation and enhanced crude oil recovery. The volume covers the gamut of topics in bacterial biosurfactants in nanostructure, including their comparison to synthetic surfactants, their interaction with microorganisms, and their biochemistry, characterization, genetics of production, bioremedial effects, and more. The volume also explores the myriad uses of bacterial biosurfactants, including in laundry detergents, cosmetics, food production, petroleum, agriculture, medicine and therapeutics, environment, metallurgy, etc. Attention to biosurfactants has been gradually increasing in recent years due to the possibility of their production through fermentation technology and their potential applications in environmental protection. Despite their numerous advantages over synthetic chemical surfactants, biosurfactants have been unable to compete with chemically synthesized surfactants due to high production costs in relation to the inefficient bioprocessing techniques, poor strain productivity, and use of costly substrates. This volume helps to identify the factors that need to be addressed to reduce the cost of production of biosurfactants.
Bacterial Capsules (Current Topics in Microbiology and Immunology #150)
by Klaus Jann Barbara JannMany bacteria, such as certain Neisseria and Haemophilus or Escherichia coli, are able to withstand the bactericidal activity of complement and phagocytes. This bacterial self protection is brought about by encapsulation. Bacterial capsules thus enable the pathogenic bacteria to survive in the host by counter action or evasion of the nonspecific host defense in the early pre immune phase of an infection. It is only in the late immune phase of the infection, when specific anticapsular antibodies are formed and enforce the host's defense system, that this protective action is overcome. Encapsulated bacteria are then killed and eliminated. Interestingly, some capsules can not or only inefficiently be handled by the immune system. The ensuing lack of antibody formation results in a prolonged susceptibility of the host to the pathogenic bacteria exhibiting such capsules. It was found that bacterial capsules consist of acidic poly saccharides. From this it followed that the role of the capsules in the interaction of encapsulated bacteria with the host may be due to the chemistry of the capsular polysaccharides. This led to intensive studies of capsular polysaccharides in many laboratories. Our increasing knowledge of the structural features of capsular polysaccharides prompted not only immuno chemical studies analyzing the interactions of these poly saccharide antigens and characterizing the epitopes, but also investigations into their biosynthesis. These studies were complemented and supported by genetic analyses. Today many interdisciplinary investigations of capsular polysaccharides are in progress.
The Bacterial Cell Surface
by S.M. Hammond P.A. Lambert Andrew RycroftIt is a common statement that because of its simplicity the bacterial cell makes an ideal model for the study of a wide variety of biological systems and phenomena. While no-one would dispute that much of our under standing of biological function derives from the study of the humble bacterium, the concept of a simple life-form would be hotly disputed by any scientist engaged in the determination of the relationship between structure and function within the bacterial cell. Bacteria are particularly amenable to intensive study; their physiology can be probed with powerful biochemical, genetical and immunological techniques. Each piece of information obtained inevitably raises as many questions as answers, and can lead to a highly confused picture being presented to the lay reader. Nowhere is this more evident than in the study of the surface layers of the bacterial cell. Examination of the early electron micrographs suggested that the bacterial cytoplasm was surrounded by some sort of semi-rigid layer, possessing sufficient intrinsic strength to protect the organism from osmotic lysis. The belief that the surface layers were rather passive led to their neglect, while researchers concentrated on the superficially more exciting cytoplasmic components. Over the last twenty years our view of the bacterial envelope has undergone extensive revision, revealing a structure of enormous complexity.
Bacterial Cell Surfaces: Methods and Protocols (Methods in Molecular Biology #966)
by Anne H. DelcourIn recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world.
The Bacterial Cell Wall
by Guntram Seltmann Otto HolstThe bacterial cell wall represents a very complex structure disconnecting the interior of single-cell organisms from the environment, thus protecting, but also enabling, them to interact with the surrounding milieu and to exchange both substances and information. Knowledge of the biochemistry of the cell wall (components) and the genetic background helps to understand their significance with regard to microbiology and immunology of bacteria. This book represents the second edition of a publication which was presented nearly 20 years ago in the German language (Die bakterielle Zellwand). Since that time our knowledge in this field has been significantly enlarged. Therefore, the manuscript had to be completely revised and updated. To maintain both the size and the introductory character of the book at least to a great extent, the authors had to restrict the presented material to that which appears basic and most important. This requirement must inevitably bring about many subjective factors. As pointed out in the first edition, the term cell wall was not taken too strictly. Since the constituents located outside the cytoplasmic membrane are frequently difficult to divide in structure, localisation, and/or function into true cell wall components and supplementary substances, they are all at least briefly mentioned.
The Bacterial Cell Wall: Methods and Protocols (Methods in Molecular Biology #2727)
by Hung Ton-ThatThis detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination of genetic, molecular, biochemical, and cytological techniques, the protocols address many fundamental questions involving the composition, biosynthesis, and regulation of bacterial peptidoglycan. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, The Bacterial Cell Wall: Methods and Protocols provides current and future researchers with a compilation of many of the most important and useful procedures in a single resource.
Bacterial Cell Wall Homeostasis: Methods and Protocols (Methods in Molecular Biology #1440)
by Hee-Jeon HongThis volume brings together the most widely used and important protocols currently being employed in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall Homeostasis cover a variety of subjects, such as: modern microscopy techniques and other biophysical methods used to characterize the subcellular structure of the bacterial cell wall; high-throughput approaches that can be used to identify all the genes and proteins that participate in the correct functioning of an organism’s cell wall; protocols for assaying individual gene products for specific cell wall functions or identify chemicals with inhibitory activity against the cell wall; and methods for analyzing the non-protein components of the cell wall and the increasing use of computational approaches for predicting and modeling cell wall related functions and processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocols emphasizes the diversity of the research taking place in bacterial cell wall homeostasis, and explains how the integration of information from across multiple disciplines is going to be essential if a holistic understanding of this important process is to be obtained.
Bacterial Cell Walls and Membranes (Subcellular Biochemistry #92)
by Andreas KuhnThis book provides an up-to-date overview of the architecture and biosynthesis of bacterial and archaeal cell walls, highlighting the evolution-based similarities in, but also the intriguing differences between the cell walls of Gram-negative bacteria, the Firmicutes and Actinobacteria, and the Archaea. The recent major advances in this field, which have brought to light many new structural and functional details, are presented and discussed. Over the past five years, a number of novel systems, e.g. for lipid, porin and lipopolysaccharide biosynthesis have been described. In addition, new structural achievements with periplasmic chaperones have been made, all of which have revealed amazing details on how bacterial cell walls are synthesized. These findings provide an essential basis for future research, e.g. the development of new antibiotics. The book’s content is the logical continuation of Volume 84 of SCBI (on Prokaryotic Cytoskeletons), and sets the stage for upcoming volumes on Protein Complexes.
Bacterial Cellulose: Synthesis, Production, and Applications
by Sher Bahadar Khan Tahseen KamalBacterial cellulose (BC) is a natural polymer produced by different microbial cells. Its unique structural, physico-chemical, mechanical, thermal, and biological properties offer much potential for use in diverse applications in the biomedical, electronics, energy, and environmental fields, among others. This text provides an overview of the synthesis, characterization, modification, and application of BC. • Discusses sources, characterization, and biosynthesis of BC • Covers composites and aerogels based on BCs • Describes development of BCs from waste and challenges in large-scale production of BCs • Explores a variety of applications such as environmental, industrial, and biomedical This book will be of great interest to researchers and industry professionals in materials science, chemical engineering, chemistry, and other related fields seeking to learn about the synthesis and application of this important material.
Bacterial Cellulose: Synthesis, Production, and Applications
by Sher Bahadar Khan Tahseen KamalBacterial cellulose (BC) is a natural polymer produced by different microbial cells. Its unique structural, physico-chemical, mechanical, thermal, and biological properties offer much potential for use in diverse applications in the biomedical, electronics, energy, and environmental fields, among others. This text provides an overview of the synthesis, characterization, modification, and application of BC. • Discusses sources, characterization, and biosynthesis of BC • Covers composites and aerogels based on BCs • Describes development of BCs from waste and challenges in large-scale production of BCs • Explores a variety of applications such as environmental, industrial, and biomedical This book will be of great interest to researchers and industry professionals in materials science, chemical engineering, chemistry, and other related fields seeking to learn about the synthesis and application of this important material.
Bacterial Cellulose: Production, Scale-up, and Applications
by Vinod Kumar Saurabh Saran Ashok Pandey Carlos Ricardo SoccolThis reference book provides updated information on the production and industrial significance of bacterial cellulose. Bacterial cellulose is a natural fiber produced by certain microbes, mainly bacteria which belong to the Acetobacter genera. The book discusses its applications in different industrial sectors, such as food, pharmaceutical, energy, and wastewater treatment. It covers the production of cellulose from conventional and renewable feedstock and includes topics such as downstream processing, characterization, and chemical modification of bacterial cellulose. FEATURES Addresses the challenges of the production technologies of bacterial cellulose up to pilot scale Discusses cost-effective green processes using agri-processing residues and medium formulation Includes efficient preparation of nanocomposites using in vitro and in vivo methods Provides the latest applications of bacterial cellulose in the food and pharmaceuticals fields Reviews the production of bacterial cellulose from conventional feedstock such as sugars and starches This book is designed for industry experts and researchers of applied microbiology, bioprocesses, and industrial microbiology.
Bacterial Cellulose: Production, Scale-up, and Applications
by Vinod Kumar Saurabh Saran Ashok Pandey Carlos Ricardo SoccolThis reference book provides updated information on the production and industrial significance of bacterial cellulose. Bacterial cellulose is a natural fiber produced by certain microbes, mainly bacteria which belong to the Acetobacter genera. The book discusses its applications in different industrial sectors, such as food, pharmaceutical, energy, and wastewater treatment. It covers the production of cellulose from conventional and renewable feedstock and includes topics such as downstream processing, characterization, and chemical modification of bacterial cellulose. FEATURES Addresses the challenges of the production technologies of bacterial cellulose up to pilot scale Discusses cost-effective green processes using agri-processing residues and medium formulation Includes efficient preparation of nanocomposites using in vitro and in vivo methods Provides the latest applications of bacterial cellulose in the food and pharmaceuticals fields Reviews the production of bacterial cellulose from conventional feedstock such as sugars and starches This book is designed for industry experts and researchers of applied microbiology, bioprocesses, and industrial microbiology.
Bacterial Cellulose: Sustainable Material for Textiles (Sustainable Textiles: Production, Processing, Manufacturing & Chemistry)
by Subramanian Senthilkannan Muthu R. RathinamoorthyThis book presents the potential of bacterial cellulose in the textile and fashion industry. Most of the earlier work on the bacterial cellulose was focused on the bio technology application of cellulose, but the recent urge for the need of a sustainable material in the fashion and textile industries identified the scope of the bacterial cellulose in this aspect. The unique feature of this book is that it relates the bio technological aspects of bacterial cellulose with the sustainable issues in the fashion industry.
Bacterial Chemosensing: Methods and Protocols (Methods in Molecular Biology #1729)
by Michael D. MansonThis volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world.
Bacterial Chromatin: Methods and Protocols (Methods in Molecular Biology #1837)
by Remus T. DameThis volume brings together a wide range of methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols aims to be useful as an up-to-date reference work for scholars in the bacterial chromatin field, those entering the field from adjacent research fields, and scientists in the eukaryotic chromatin field.
Bacterial Chromatin: Methods and Protocols (Methods in Molecular Biology #2819)
by Remus T. DameThis second edition brings together new and updated methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols, Second Edition aims to be a useful up-to-date reference work for researchers currently in the field and to those entering the field.
Bacterial Chromatin: Methods And Protocols (Methods in Molecular Biology #1837)
by Remus T. Dame Charles J. DormanThe birth and the development of molecular biology and, subsequently, of genetic engineering and biotechnology cannot be separated from the advancements in our knowledge of the genetics, biochemistry and physiology of bacteria and bacter- phages. Also most of the tools employed nowadays by biotechnologists are of bacterial (or bacteriophage) origin and the playground for most of the DNA manipulations still remains within bacteria. The relative simplicity of the bacterial cell, the short gene- tion times, the well defined and inexpensive culturing conditions which characterize bacteria and the auto-catalytic process whereby a wealth of in-depth information has been accumulated throughout the years have significantly contributed to generate a large number of knowledge-based, reliable and exploitable biological systems. The subtle relationships between phages and their hosts have produced a large amount of information and allowed the identification and characterization of a number of components which play essential roles in fundamental biological p- cesses such as DNA duplication, recombination, transcription and translation. For instance, to remain within the topic of this book, two important players in the or- nization of the nucleoid, FIS and IHF, have been discovered in this way. Indeed, it is difficult to find a single fundamental biological process whose structural and functional aspects are better known than in bacteria.
Bacterial Chromatin (Proceedings in Life Sciences)
by Claudio O. Gualerzi Cynthia L. PonThis book brings together various contributions aimed at the elucida tion of the structural and functional organization of the bacterial nucleoid. Most of these papers, spanning the fields of physical chemistry through biochemistry to genetics, were presented at the session on bac terial chromatin during the Symposium "Selected topics on chromatin structure and function" held at the University of Camerino, Italy, at the end of May 1985. Times when the bacterial DNA was regarded as "naked" or, at most, complexed with polyamines, and when the absence of histones and organized chromatin was considered to be a distinct feature of the pro karyotic cell, now appear remote. Our concepts of how DNA is packaged in bacteria are changing rapidly. Studies on the structure of the bacterial nucleoid are not new. Recently, however, investigations in this field have flourished again, leading to some important contributions such as the elucidation of the three-dimensional structure of what appears to be the major protein constituent of the bacterial nucleoid or the development of methods to titrate the extent of DNA supercoiling within the bacterial cell.
Bacterial Circadian Programs
by Jayna L. Ditty Shannon R. Mackey Carl H. JohnsonSince the discovery of circadian rhythms in cyanobacteria in the late 1980s, the field has exploded with new information. The cyanobacterial model system for studying circadian rhythms, "Synechococcus elongatus", has allowed a detailed genetic dissection of the bacterial clock due to the methods currently available in molecular, structural, and evolutionary biology. This book addresses multiple aspects of bacterial circadian programs: the history and background of the cyanobacteria and circadian rhythms in microorganisms, the molecular basis, structure, and evolution of the circadian clock, entrainment of the oscillator with the environment and the control of downstream processes by the clock, the demonstration of adaptive significance and the prokaryotic clock’s remarkable stability, and mathematical and synthetic oscillator models for clock function. Experts in the field provide a timely and comprehensive review and a stepping-stone for future work on this amazing group of microorganisms and timing.