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Bacterial Type III Protein Secretion Systems (Current Topics in Microbiology and Immunology #427)
by Samuel Wagner Jorge E. GalanOne of the most exciting developments in the field of bacterial pathogenesis in recent years is the discovery that many pathogens utilize complex nanomachines to deliver bacterially encoded effector proteins into eukaryotic and prokaryotic target cells to modulate a variety of cellular functions for the pathogen’s benefit. These protein-delivery machines include the type III secretion system (T3SS), which is widespread in nature and encoded not only by bacteria pathogenic to vertebrates or plants, but also by bacteria that are symbiotic to plants or insects. Because they are essential virulence factors for many important human pathogens, these systems are emerging as a prime target for the development of new-generation, anti-infective drugs. This book reviews our current understanding of these intriguing injection machines as well as of the closely related T3SS that serves in flagella assembly. Individual chapters focus on regulation, assembly, structure, and function of the type III secretion machine and on the evolution of the secreted effector proteins. Given its scope, this book will appeal to a broad readership, including researchers and teachers in the fields of infectious diseases, host pathogen interactions, plant and animal pathogenesis, and symbiosis.
Bacterial Vaccines: Methods and Protocols (Methods in Molecular Biology #2414)
by Fadil Bidmos Janine Bossé Paul LangfordThe importance of vaccines to combat bacterial diseases cannot be overstated. Methods used in the development and testing of these vaccines are evolving rapidly as a direct consequence of the availability of advanced technologies. This volume will cover methods developed in the last decade, the usage of which are enabling the development of cheaper, cost-effective and structurally stable vaccines for global use. Chapters cover in silico analytical methods such as reverse vaccinology and machine learning; low-energy electron irradiation for the generation of inactivated bacterial vaccines; methods for assessment of OMV/GMMA quality and stability; and controlled human infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Bacterial Vaccines: Methods and Protocols is a valuable resource for novice and expert researchers interested in learning more about this important and constantly evolving field.
Bacterial Virulence: Methods and Protocols (Methods in Molecular Biology #2427)
by Ohad Gal-MorThis detailed volume explores the field of bacterial virulence and the effort to understand how microbial interaction with a host results in the pathology of a specific disease. This collection of selected protocols includes advanced molecular biology and bioinformatics methods, cell culture and organoid models of infection, as well as in vivo infection models that are useful to study the interaction of pathogens with plants, insects, avian, and mammalian hosts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and beneficial, Bacterial Virulence: Methods and Protocols serves as an ideal guide for researchers seeking to promote and further develop the exciting and continuously evolving field of bacterial virulence.Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Bacterial Virulence: A Conceptual Primer
by Anthony William MaressoThis textbook introduces in an engaging way the fundamentals of how pathogenic bacteria interact with, and are virulent within, the human host. To inspire and educate the next generation of microbe hunters, the author, Microbiologist and Scientist Anthony William Maresso, integrates the major findings of the field into a single, easy-to-understand volume emphasizing a molecular appreciation of the concepts underlying bacterial infectious diseases. The work explores such themes as the history of Microbiology, bacterial structure and physiology, bacterial toxins, secretion systems, and adhesins, the host immune system and its battle with bacteria, biofilms, sepsis, and technologies/techniques to the present day. Fully illustrated in concept and packed with idea-provoking challenges highlighting “out-of-the-box” thinking, the work moves beyond being just a review of the scientific literature intent on equipping the next generation of Microbiologists and their teachers with the knowledge to confront, and hopefully one day defeat, the insidious microbes which undermine human health. This textbook is a resource for undergraduate, graduate, and medical students, as well as other health-oriented learners, postdoctoral scholars, basic scientists, and professors intent on expanding their knowledge of bacterial infection and virulence mechanisms.
Bacterial Virulence Factors and Rho GTPases (Current Topics in Microbiology and Immunology #291)
by Patrice Boquet E. LemichezPathogenic bacteria for human and animals have developed sophisticated weapons, termed virulence factors, to ensure their replication and persistence into their hosts. The authors in this volume show a synthesis on how the various host cellular Rho GTPases activities are manipulated by bacteria to fulfil their virulence.
Bacterial Volatile Compounds as Mediators of Airborne Interactions
by Choong-Min Ryu Laure Weisskopf Birgit PiechullaThis book covers the fundamentals of bacterial volatile-mediated communication with other organisms, starting with the biosyntheses of volatile organic compounds (VOC), interactions with plants and animals, interactions with microbes, tools for data analysis, and their applications. With this foundation in place, the book subsequently focuses on understanding the effect of bacterial volatiles on plant growth promotion, discusses plant immunity, and lastly shares insights into future research directions. The book is divided into fourteen-in-depth chapters, each of which is designed to enrich readers’ understanding of bacterial volatile compounds’ functions and various applications.The pivotal roles of bacterial volatile compounds make this book essential reading for scientists and students of all biological disciplines seeking to fully understand microorganism responses and environmental adaptations. In addition to its value as a fundamental book for graduate students, it offers a clearly structured reference guide for all individuals working in microbiology.
Bacterial Wilt Disease: Molecular and Ecological Aspects
by Philippe Prior Caitilyn Allen John ElphinstoneJointly published with INRA, Paris.Bacterial wilt, caused by Ralstonia solanacearum, is a very destructive plant disease that attacks over 450 different species, including many of the most important economic crop plants. Often endemic, the bacterium transmits through the soil, penetrates the plant root system and eventually causes irreversible wilting and death. This book summarizes the current information on bacterial wilt for both the basic research community and for concerned professionals who are faced with the disease in the field, offering the latest approaches to diagnosis and control of the disease. Emphasis is placed on integrated and biologically sustainable control methods. Also presented is the most recent genetic/biochemical research exploring the interaction between the bacterium and its plant host at the molecular level.
Bacteriocins
by Qing GuThis book intends to report the new results of the study of bacteriocins, from basic research to application fields. It mainly introduces the biological characteristics of bacteriocins, the relationship between their structure and function, the antibacterial mode of action, and their application as antibacterial agents in food industry, medical care, and other areas, especially their application potential in human health. This book can be used as a reference book for researchers, undergraduates, and graduated students in the professional fields of food science and engineering, bioengineering, medicine, and agriculture.
The Bacteriocins (Molecular Biology, Biochemistry and Biophysics Molekularbiologie, Biochemie und Biophysik #11)
by Peter ReevesIn recent years bacteriocins, especially colicins, have become widely known to molecular biologists as proteins with peculiar ways of killing bacteria. These same bacteriocins have been known for a long time to bacteriology for their unusual activity spectra and enormous variety. In this monograph I have attempted to bring together our detailed knowledge of those few bacteriocins which have already re ceived attention from molecular biologists, and our less detailed hut extensive knowledge of the variety of bacteriocins which exist. The field has been reviewed in whole or in part, by several authors [FREDERICQ, 1957, 1964, 1965 (2); IVANOVICS, 1962; HAMON, 1965; REEVES, 1965 (2)]. These reviews have been very useful to the author, and readers will find further references in them, and sometimes alternative viewpoints. We have already referred to bacteriocins as proteins, and in doing so have ex cluded many more complex antibacterial agents which resemble bacteriophages or their tails. In the author's view, these phage-like particles are probably not bacterio cins, but many authors include them within the definition; the more restrictive de finition used here has meant omitting discussion of some excellent studies on what the present author would term defective bacteriophages. In the first chapter we look at the discovery of bacteriocins and an outline of their classification. With this background we can discuss in Chapters 2 to 6 the chemistry, genetics and mode of action of the more intensively studied bacteriocins.
Bacteriocins: Ecology and Evolution
by Margaret A. Riley Milind A. ChavanMicrobes produce an extraordinary array of defense systems. This book tells the fascinating story about the evolutionary histories of bacteriocins and the ecological roles of these biological weapons in microbial communities. The book makes compelling reading for a multi-faceted scientific audience, including those working in the fields of biodiversity and biotechnology, notably in the human and animal health domain.
Bacteriocins, Microcins and Lantibiotics (Nato ASI Subseries H: #65)
by Richard James Claude Lazdunski Franc Pattus
Bacteriocins of Lactic Acid Bacteria: Microbiology, Genetics and Applications
by Luc De Vuyst Erick J. VandammeAs antibacterial compounds, bacteriocins have always lived in the shadow of those medically important, efficient and often broad-spectrum low-molecular mass antimicrobials, well known even to laypeople as antibiotics. This is despite the fact that bacteriocins were discovered as early as 1928, a year before the penicillin saga started. Bacteriocins are antimicrobial proteins or oligopeptides, displaying a much narrower activity spectrum than antibiotics; they are mainly active against bacterial strains taxonomically closely related to the producer strain, which is usually immune to its own bacteriocin. They form a heterogenous group with regard to the taxonomy of the producing bacterial strains, mode of action, inhibitory spectrum and protein structure and composition. Best known are the colicins and microcins produced by Enterobacteriaceae. Many other Gram-negative as well as Gram-positive bacteria have now been found to produce bacteriocins. In the last decade renewed interest has focused on the bacteriocins from lactic acid bacteria, which are industrially and agriculturally very important. Some of these compounds are even active against food spoilage bacteria and endospore formers and also against certain clinically important (food-borne) pathogens. Recently, bacteriocins from lactic acid bacteria have been studied intensively from every possible scientific angle: microbiology, biochemistry, molecular biology and food technology. Intelligent screening is going on to find novel compounds with unexpected properties, just as has happened (and is still happening) with the antibiotics. Knowledge, especially about bacteriocins from lactic acid bacteria, is accumulating very rapidly.
Bacteriology of Humans: An Ecological Perspective
by Michael Wilson1st Prize, 'New Authored Books' category, Royal Society of Medicine and Society of Authors Medical Book Awards 2008 “Overall, I am impressed by the up-to date information content and structure provided in Bacteriology of Humans. It is truly an ecological perspective helpful for undergraduate/graduate majors in microbiology and immunology.” –American Society for Microbiology, June 2009 "Wilson provides the reader with an up-to-date, comprehensive census of the indigenous microorganisms that inhabit the human body and in so doing contributes significantly to this rapidly advancing area of study. The narrative is clearly written; the index is excellent; there are numerous bibliographic citations. Each chapter is rich with tables, diagrams, color micrographs, and charts … Highly recommended." –Choice Reviews "This comprehensive, yet accessible text... is an excellent and informative reference book… it should be on the shelf of every major science and medical library. The content, organization, and presentation make this book a unique resource." –Doody's Book Reviews Until recently, the indigenous microbiota of humans has been a relatively neglected area of microbiology with most attention being focused on those microbes that cause disease in humans, rather than on those that co-exist with us in the disease-free state. However, in the past decade research has shown that not only is the indigenous microbiota involved in protecting humans from exogenous pathogens but it is also involved in our development and nutrition. Consequently, interest has grown substantially among health professionals and scientists in analyzing and understanding these microbial (largely bacterial) communities. This comprehensive, yet accessible text provides an up-to-date guide to the development, composition and distribution of indigenous microbial communities of humans. With the aid of abundant colour figures, diagrams, tables and maps, it establishes links between the physicochemical factors prevailing at an anatomical site and the types of microbes to be found there. The book includes an introduction to the human-microbe symbiosis as well as an in-depth look at the main systems and organs of the human body that have an indigenous microbiota. Each chapter includes a list of references for further study. This is an excellent and informative reference book that is useful to anyone with an interest in microbiology, medical microbiology, microbial ecology, infectious diseases, immunology, human biology, medicine, dentistry, nursing, health sciences, biomedical sciences or pharmacy – it should be on the shelf of every major science and medical library. Hallmark Features: Provides a comprehensive, yet accessible, reference book on the human microbiota Lavishly illustrated with colour figures, diagrams, tables and maps Each chapter provides a list of references to promote further study Each chapter contains links to key websites Offers an ecological approach that explains why certain organisms are associated with a particular anatomical site
Bacteriophage Applications - Historical Perspective and Future Potential (SpringerBriefs in Biochemistry and Molecular Biology)
by Jessica Nicastro Shirley Wong Zahra Khazaei Peggy Lam Jonathan Blay Roderick A. SlavcevThis book explores key applications of phage biotechnology and reviews recent advances in phage display technologies. The applications covered were selected on the basis of their significance and representativeness in the field. The small size and enormous diversity of bacteriophages make them ideal candidates for numerous applications across many industries. Since the discovery of phages and the advent of phage display systems, considerable attention has been focused on the development of novel therapeutic and industrial applications. Recent studies combine the genomic flexibility of phages with phage display systems in order to generate modified phages for targeted delivery.
Bacteriophage Therapy: From Lab to Clinical Practice (Methods in Molecular Biology #2734)
by Joana Azeredo Sanna SillankorvaThis highly anticipated second edition details cutting-edge experimental approaches for bacteriophage research. This comprehensive volume details new and updated experimental approaches for isolating, formulating, and engineering bacteriophage medicinal products. Authored by leading experts and written in the highly successful Methods in Molecular Biology series format, each chapter includes introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and valuable tips for troubleshooting and avoiding known pitfalls. Whether you are a seasoned scientist or a newcomer, Bacteriophage Therapy: From Lab to Clinical Practice, Second Edition provides essential tools and knowledge to advance bacteriophage research and its translation into innovative medicinal products and clinical therapies.
Bacteriophage Therapy: From Lab to Clinical Practice (Methods in Molecular Biology #1693)
by Joana Azeredo and Sanna SillankorvaThis volume details the experimental approaches suitable for isolating and characterizing bacteriophages to formulating bacteriophage medicinal products and clinical application. Chapters guide readers through regulatory compliance and safety aspects of bacteriophage therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacteriophage Therapy: From Lab to Clinical Practice aims to ensure successful results in the further study of this vital field.
The Bacteriophages
by . Stephen AbedonThis authoritative, timely, and comprehensively referenced compendium on the bacteriophages explores current views of how viruses infect bacteria. In combination with classical phage molecular genetics, new structural, genomic, and single-molecule technologies have rendered an explosion in our knowledge of phages. Bacteriophages, the most abundant and genetically diverse type of organism in the biosphere, were discovered at the beginning of the 20th century and enjoyed decades of used as anti-bacterial agents before being eclipsed by the antibiotic era. Since 1988, phages have come back into the spotlight as major factors in pathogenesis, bacterial evolution, and ecology. This book reveals their compelling elegence of function and their almost inconceivable diversity. Much of the founding work in molecular biology and structural biology was done on bacteriophages. These are widely used in molecular biology research and in biotechnology, as probes and markers, and in the popular method of assesing gene expression.
The Bacteriophages: Volume 1 (The Viruses)
by Richard CalendarIt has been 10 years since Plenum included a series of reviews on bacte riophages, in Comprehensive Virology. Chapters in that series contained physical-genetic maps but very little DNA sequence information. Now the complete DNA sequence is known for some phages, and the se quences for others will soon follow. During the past 10 years two phages have come into common use as reagents: A phage for cloning single copies of genes, and Ml3 for cloning and DNA sequencing by the dideoxy termi nation method. Also during that period the use of alternative sigma fac tors by RNA polymerase has become established for SPOl and T4. This seems to be a widely used mechanism in bacteria, since it has been implicated in sporulation, heat shock response, and regulation of nitro gen metabolism. The control of transcription by the binding of A phage CII protein to the -35 region of the promoter is a recent finding, and it is not known how widespread this mechanism may be. This rapid progress made me eager to solicit a new series of reviews. These contributions are of two types. Each of the first type deals with an issue that is exemplified by many kinds of phages; chapters of this type should be useful in teaching advanced courses. Chapters of the second type provide comprehensive pictures of individual phage families and should provide valuable information for use in planning experiments.
Bacteriophages: Methods and Protocols, Volume IV (Methods in Molecular Biology #1898)
by Martha R. J. Clokie Andrew Kropinski Rob LavigneThis book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag® II purifications; and also application driven chapters including 'duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems’. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both established and novice phage scientists.
Bacteriophages
by John DouglasThe contribution of bacteriophages to the development of modern biology cannot be overestimated yet, sixty years after their discovery, they are as remote and mysterious to many scientists as they are to most laymen. This book endeavours to remedy the situation: an attempt has been made to provide, in readily comprehensible form, a nucleus of information essential to anyone embarking on the study of bacteriophages or using them in their work for the first time. It shows the range of bacteriophage structure and behaviour; it illustrates the role of bacteriophage in molecular biology; it surveys the current state of the art; it presents the medical and industrial aspects. Some simple experimental procedures are given in sufficient detail for the beginner to attempt them successfully. Other, more sophisticated pro cedures are presented so as to impart a feeling of intimate reality without dazzling the reader with technical complexity. I hope that young readers will forgive me for assuming that they have some knowledge of bacteria, nucleic acids, antibodies and isotopes. Likewise I would ask mature workers to excuse the omission of cherished specialities. To have included all these, valuable though they are, might have put this book beyond the re ach of the phage-novices for whom it is intended. Specific references, save a few of particular interest, have been omitted. Other books on bacteriophage v vi BACTERIOPHAGES provide them in abundance.
Bacteriophages: Biology, Technology, Therapy (pdf) (Advances In Molecular And Cellular Microbiology Ser. #15)
by David R. Harper Stephen T. Abedon Benjamin H. Burrowes Malcolm L. McConville
Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions (Methods in Molecular Biology #501)
by Martha R.J. Clokie and Andrew M. KropinskiRanging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.
Bacteriophages: Methods and Protocols, Volume 2: Molecular and Applied Aspects (Methods in Molecular Biology #502)
by Martha R.J. Clokie and Andrew M. KropinskiRanging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.
Bacteriophages: Methods and Protocols, Volume 3 (Methods in Molecular Biology #1681)
by Martha R.J. Clokie, Andrew M. Kropinski and Rob LavigneThis book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III is a valuable resource for both established and novice phage scientists.
Bacteriophages: Practical Applications for Nature's Biocontrol
by Sabah A.A. Jassim Richard G. LimogesBacteriophages: Practical Applications for Nature's Biocontrol' presents the latest information on uses in healthcare settings as well as animal husbandry, management and care of farm animals by using enhanced phages to replace antibiotics for growth promotion in animal feed or to prevent, control and treat disease in animals. The book will provide an overview of the function of phages and what researchers need to know, from phage hunting to laboratory design, management, production and application using different tools and methods. These key aspects will be discussed through a series of dedicated chapters, with topics covering auditing, validation, data analysis, microbial identification, culture media, and contamination control, etc.