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Bacteriophages: Methods and Protocols (Methods in Molecular Biology #2738)
by Ebenezer TumbanThis volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small and jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture and molecular techniques, protocols for isolation, interaction of bacteriophage proteins with host cells, and how to use bacteriophages to transfer foreign genetic elements to bacterial strains. In addition to the above, chapters feature the application of bacteriophages/bacteriophage-derived products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips (in the Notes section) on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacteriophages: Methods and Protocols aims to ensure successful results in further study of this vital field.
Bacteriophages as Drivers of Evolution: An Evolutionary Ecological Perspective
by Stephen T. AbedonThis monograph emphasizes the many facets of bacterial evolution as impacted by bacterial interactions with phages, as well as, to a lesser degree, the evolutionary impact of phages on other organisms, including other phages. The book starts with a general overview of bacteriophages. Topics discussed in detail include but are not limited to mutagenesis, migration, natural selection and genetic drift as the drivers of evolution as well as an extensive discussion from the author’s unique perspective on phage ecology.
Bacterium (Labelled diagram) (tactile)
by Sheffield Vi ServiceThis is a cut-away view of a bacterium. It has labelled leader lines pointing to parts of the bacterium.
Bacterium (Unlabelled black & white diagram) (tactile)
by Sheffield Vi ServiceThis is an unlabelled, cut-away view of a bacterium. It has leader lines pointing to parts of the bacterium, which can be labelled.
Bacterium (Unlabelled colour diagram) (tactile)
by Sheffield Vi ServiceThis is an unlabelled, coloured cut-away view of a bacterium. It has leader lines pointing to parts of the bacterium, which can be labelled.
Bactine / Giganthomachy / Pills (Storycuts)
by Donald Ray PollockIn 'Bactine', a toothless layabout takes a break from nursing his crippled uncle and spends the evening abusing solvents with an acquaintance. The fumes only serve to exacerbate his dismay, though, and a trip to an all-night coffee shop causes some unpleasant recollections to surface.In 'Giganthomachy', Teddy and William are two nine year-old boys who like to argue over whether they'll play nuclear war or Vietnam games in the yard. William's more fantastic suggestions rile Teddy, who suffers enough deranged fantasy when he retreats inside every evening.In 'Pills', Bobby and Frankie take a notion to leave Knockemstiff for California and a new life amongst the film stars. But the four stolen bottles of pharmaceutical grade speed they'd intended to fund the trip with prove mighty alluring, and the road out of the township grows ever more elusive.Part of the Storycuts series, these three short stories were previously published in the collection Knockemstiff.
Baculovirus: Methods and Protocols (Methods in Molecular Biology #2829)
by Manon M. J. CoxThis detailed volume provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus stocks or to quantify product produced, and it concludes with alternative uses of either baculovirus or insect cells as tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Baculovirus: Methods and Protocols serves as an ideal guide for researchers looking to overcome some of the limitations associated with the early baculoviral vectors and cell lines.
Baculovirus and Insect Cell Expression Protocols (Methods in Molecular Biology #1350)
by David W. MurhammerThe third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
Baculovirus and Insect Cell Expression Protocols (Methods in Molecular Biology #388)
by David W. MurhammerBaculovirus Expression Protocols, Second Edition, provides the detailed steps required to perform the techniques involved with the use of baculoviruses and insect cell culture and discusses problems that may be encountered. This newly-revised and updated edition provides a cutting edge step-by-step guide for biochemists, molecular biologists, biochemical engineers and others using the BEVS and/or insect cells for producing recombinant proteins. Furthermore, Baculovirus Expression Protocols, Second Edition, provides assistance to scientists and engineers interested in developing and producing baculovirus insecticides. Authors list all available insect cell lines and provide methods for isolating new cell lines. The procedures involved in producing products, both at laboratory scale and large scale, are discussed, as well as the production in insect larvae. It is hoped that this volume will not only aid the user in successfully completing the tasks described herein, but will also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture. Timely and thorough, this volume will become an essential reference for anyone using baculoviruses and insect cell culture.
Baculovirus Expression Protocols (Methods in Molecular Biology #39)
by Christopher D. RichardsonBaculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.
The Baculovirus Expression System: A laboratory guide
by Linda KingThe decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora tories requiring assistance to find the elusive polyhedrin-negative virus containing their favourite gene. We have also organized two expression system workshops and from the manuals we wrote for these, it seemed a logical progression to extend them into book form. We appreciate that those who are 'old-hands' at the baculovirus expression system may have differing views on some of our procedures, but the methods in this book are presented in the light of our own experiences in the laboratory and from our practical workshops, and we hope that the book will be especially useful to those new to the system. The first three chapters give the background information to the baculovirus expression system, and includes advice on how to choose the right transfer vector and discusses the various methods that are available to select recombinant viruses. The practical chapters concentrate on those aspects which are novel to the baculovirus system (insect cell culture, virus amplification and titration, etc. ) and, in general, leave the standard molecular biological techniques to the other excellent laboratory manuals that are available. However, for completeness sake and to avoid constant reference to other manuals, we have included brief details of some standard techniques where they are integral to the success of the baculovirus protocols.
The Baculoviruses (The Viruses)
by Lois K. MillerThe past decade has witnessed an explosion of information on the molecular biology of insect viruses and a frenzy of activity in applying this information to medicine and agriculture. Genetically engineered baculoviruses are presently being tested for commercial use as pesticides, and the study of such viruses is also revealing remarkable insights into basic cellular processes such as apoptosis. This comprehensive volume provides readers with knowledge of basic and applied baculovirology so that current literature in the field can be appreciated.
Bad: A gripping, dark and outrageously funny thriller (Mad, Bad and Dangerous to Know Trilogy)
by Chloé EspositoThe brand-new summer sizzler . . . ____________She stole the life she wanted. Now someone wants to steal it back . . . Alvie Knightly may be waking up in the Ritz, but her life is no bed of roses.Firstly, she has the mother of all hangovers.Secondly, her beautiful, spoiled twin sister Beth has just been found dead in Sicily - and the police want Alvie for questioning.And thirdly, Alvie's hot new boyfriend has vanished with every penny of the millions they stole from Beth.But he picked the wrong girl to mess with.Alvie will pursue her ex to Rome in a game of cat and mouse that only one of them can survive.Hell hath no fury like a woman scorned . . .But can Alvie get revenge before her crimes catch up with her? ____________What do readers think?'I couldn't put it down' 'This is the most fun you'll have with a book this summer. I loved it.' 'I fell straight into the story. I loved the chase, the thrill, the ending . . . just all of it.''A glam read, perfect for the beach'_________Loved Bad? Why not read more about Alvie in her other book, Mad?
Bad Actors: The Instant #1 Sunday Times Bestseller
by Mick Herron*Discover The Secret Hours, the gripping new thriller from Mick Herron and an unmissable read for Slough House fans**Now a major TV series starring Gary Oldman*THE INSTANT #1 SUNDAY TIMES BESTSELLER'A pitch-perfect espionage thriller' Sunday TimesIn MI5 a scandal is brewing and there are bad actors everywhere.A key member of a Downing Street think-tank has disappeared without a trace. Claude Whelan, one-time First Desk of MI5's Regent's Park, is tasked with tracking her down. But the trail leads straight back to Regent's Park HQ itself, with its chief, Diana Taverner, as prime suspect. Meanwhile her Russian counterpart has unexpectedly shown up in London but has slipped under MI5's radar.Over at Slough House, the home for demoted and embittered spies, the slow horses are doing what they do best: adding a little bit of chaos to an already unstable situation.In a world where lying, cheating and backstabbing is the norm, bad actors are bending the rules for their own gain. If the slow horses want to change the script, they'll need to get their own act together before the final curtain.*Includes the short story Standing by the Wall: A Slough House Interlude*'The foremost living spy novelist in the English language' New Statesman'This is entertainment of the highest class' Literary Review'The man is a genius' The Spectator
Bad Acts and Guilty Minds: Conundrums of the Criminal Law (Studies in Crime and Justice)
by Leo KatzWith wit and intelligence, Leo Katz seeks to understand the basic rules and concepts underlying the moral, linguistic, and psychological puzzles that plague the criminal law. "Bad Acts and Guilty Minds . . . revives the mind, it challenges superficial analyses, it reminds us that underlying the vast body of statutory and case law, there is a rationale founded in basic notions of fairness and reason. . . . It will help lawyers to better serve their clients and the society that permits attorneys to hang out their shingles."—Edward N. Costikyan, New York Times Book Review
Bad Acts and Guilty Minds: Conundrums of the Criminal Law (Studies in Crime and Justice)
by Leo KatzWith wit and intelligence, Leo Katz seeks to understand the basic rules and concepts underlying the moral, linguistic, and psychological puzzles that plague the criminal law. "Bad Acts and Guilty Minds . . . revives the mind, it challenges superficial analyses, it reminds us that underlying the vast body of statutory and case law, there is a rationale founded in basic notions of fairness and reason. . . . It will help lawyers to better serve their clients and the society that permits attorneys to hang out their shingles."—Edward N. Costikyan, New York Times Book Review
Bad Acts and Guilty Minds: Conundrums of the Criminal Law (Studies in Crime and Justice)
by Leo KatzWith wit and intelligence, Leo Katz seeks to understand the basic rules and concepts underlying the moral, linguistic, and psychological puzzles that plague the criminal law. "Bad Acts and Guilty Minds . . . revives the mind, it challenges superficial analyses, it reminds us that underlying the vast body of statutory and case law, there is a rationale founded in basic notions of fairness and reason. . . . It will help lawyers to better serve their clients and the society that permits attorneys to hang out their shingles."—Edward N. Costikyan, New York Times Book Review
Bad Acts and Guilty Minds: Conundrums of the Criminal Law (Studies in Crime and Justice)
by Leo KatzWith wit and intelligence, Leo Katz seeks to understand the basic rules and concepts underlying the moral, linguistic, and psychological puzzles that plague the criminal law. "Bad Acts and Guilty Minds . . . revives the mind, it challenges superficial analyses, it reminds us that underlying the vast body of statutory and case law, there is a rationale founded in basic notions of fairness and reason. . . . It will help lawyers to better serve their clients and the society that permits attorneys to hang out their shingles."—Edward N. Costikyan, New York Times Book Review
A Bad and Stupid Girl: A Novel (Michigan Literary Fiction Awards)
by Jean McGarrySiri is a legacy admission, rich and spoiled and destined to flunk out of her freshman year at college. Esther, her roommate, is a scholarship student from humble means, brilliant and driven to succeed. Brought together by chance, the girls soon become partners in a struggle to find their way in a world where neither Esther’s brains nor Siri’s beauty is enough. Never having been forced to work hard at anything, Siri must rely on Esther to teach her to learn and attend class. But as Siri wakes from her dream world to discover the life of the mind, Esther begins shedding her rational bonds to explore the mysteries of the soul. For both, some of the most devastating lessons in the attainment of worldly knowledge come from love. Deadpan funny and bittersweet, A Bad and Stupid Girl is above all else a moving portrait of two friends helping each other to uncover the potential splendor of their lives. Jean McGarry is the author of six previous books of fiction: Airs of Providence, The Very Rich Hours, The Courage of Girls, Home at Last, Gallagher's Travels, and Dream Date. She is a professor of fiction at The Writing Seminars at Johns Hopkins University. A Bad and Stupid Girl is her third novel. “Jean McGarry's novel is a lovely locket of a book, with the picture inside not at all faded. Focused in close-up, succinct and convincing, it's a story about friendship and maturation, and about how our studies, alone, do not define us.” —Ann Beattie “Jean McGarry’s A Bad and Stupid Girl is an uncommonly Good and Bright-Indeed Novel, sharply written from start to finish and entertaining as Hell.” —John Barth “Everything in life is arbitrary yet must be over-determined in literature. Jean McGarry knows how to tell a persuasive tale illuminating these truths.” —Harold Bloom
The Bad Angel Brothers
by Paul TherouxA deliciously dark, atmospheric novel about family and brotherhood from one of America's most distinctive writersThere's sibling rivalry and then there's the relationship of brothers Cal and Frank Belanger.Enemies since childhood, the small town of Littleford just isn't big enough to hold them both. So, Cal strikes out for the world's wild places - a gifted geologist in search of gold and other precious minerals - leaving Frank to develop a successful career as the town's lawyer, fixer and local hero.But when Cal, newly rich and newlywed, returns to the town of his birth, Frank gives him the opposite of a brotherly welcome, leading to a series of betrayals and reprisals culminating in the ultimate plan: murder.A riveting tale of adventure, betrayal and the true cost of family bonds, The Bad Angel Brothers is a remarkable novel from one of American's most distinctive writers.'Laden with jealousy, betrayal and a mythic lust for vengeance' The New York Times'One of the most accomplished and worldly-wise writers of his generation' The Times
The Bad Apple: A powerful saga of surviving and loving against the odds
by Rosie GoodwinA mother and son on the run. A new beginning. A past they cannot escape. An unforgettable saga of love, escapism and intrigue from the bestselling author of No One's Girl, Mothering Sunday and The Little Angel, The Bad Apple is sure to leave you hungry for more. Perfect for fans of Shelia Newberry and Dilly Court.'Rosie Goodwin is a born storyteller - she'll make you cry, she'll make you laugh, but most of all you'll care for her characters and lose yourself in her story. An author destined for the top' - Jeannie JohnsonLouise Hart has endured a great deal of pain from her callous husband Paul, but one cold December night in 1959 she can take no more. She and her son Davey escape their Coventry high-rise flat and flee to Tanglewood, the dilapidated ivy-clad mansion where her mother Dolly runs an animal sanctuary. Miserable and frightened, Louise longs to turn her back on the past, and create a happy new life for Davey. But Paul is in trouble with men more dangerous than himself. Unable to pay his debts, he blackmails Louise and those who love her, keeping a shadowy presence in their lives. Even the arrival of Charlie Fox, a stranger who becomes a true friend, cannot guarantee that the future will be safe...What Amazon readers are saying about The Bad Apple:'Rosie's writing style enables you to envision the characters so clearly they seem to be truly alive. She hooks you in and you can't get loose from her gripping words! This story has surprising twists also. It makes you laugh, it makes you cry, it makes you think. I'm a fairly fast reader, but it took 1/2 a box of tissues and nearly an hour for me to get through 3 pages near the end of this book because I couldn't stop sobbing!' 'This is the first book I have read by Rosie Goodwin and to say it's fantastic is an understatement! I couldn't put it down and it had me reaching for the tissues on more than one occasion. It's the most believable and touching book I have ever read'
Bad Apple
by null Alice Hunter'Bad Apple is a must read for lovers of thrillers!’ ⭐⭐⭐⭐⭐ What would you do if you found out the man you loved was rotten to the core? Prepare to be hooked by Alice Hunter’s addictive new novel – so shocking it should come with a warning… Trusted officer. Family man. Monster. Becky Lawson’s life has been shattered. When she discovered her husband, John – a trusted policeman – was a monster, she reported him. But her faith in the system was crushed when it didn't lead to any charges or consequences. Now, John lives freely with a new girlfriend and her young daughter, while Becky battles guilt over missing the obvious signs. Determined for justice, Becky hunts him down. But John wants her silenced – at any cost. Becky knows only one of them can survive, and she’ll do anything to make sure it’s her. Becky must tread carefully though, because John isn’t the only bad apple lurking in the shadows… A gripping psychological suspense about betrayal, courage, and the darkness that can hide behind a seemingly trustworthy façade. Perfect for fans of K.L. Slater, B.A. Paris and the Netflix hit TV series You. ___________ READERS ARE GRIPPED BY BAD APPLE! ‘I buzzed through this book in one sitting!’ Reader Review, ⭐⭐⭐⭐⭐ ‘An addictive read, filled with suspense and clever twists right to the unexpected ending.' Reader Review, ⭐⭐⭐⭐⭐ ‘I flew through this…a five-star read.’ Reader Review, ⭐⭐⭐⭐⭐ ‘This book was extremely captivating…so many twists and turns that kept me on the edge of my seat!’ Reader Review, ⭐⭐⭐⭐⭐ ‘The book explores themes such as trust and deception with everything else in between…I found myself eagerly flicking the page to see what would happen next!’ Reader Review, ⭐⭐⭐⭐⭐ ‘This was one of my favourite thriller books I have read…absolutely fantastic!’ Reader Review, ⭐⭐⭐⭐⭐ ‘A brilliant, twisty police procedural story.’ Reader Review, ⭐⭐⭐⭐⭐
Bad Apple: A Novel
by Zoje Stage"Beautifully written, intelligent, uncompromising - highly recommended." Sophie HannahSeven-year-old Hanna has never spoken a word.She is a sweet but silent angel in the eyes of her adoring father, but with Mummy, things are different. Suzette loves her daughter but difficulties with babysitters and teachers over the years have put a strain on their relationship and her sanity. Then Hanna speaks for the first time, to Suzette alone, and what she says is chilling. Suzette wants to write it off as a scary joke, but she’s becoming increasingly frightened by Hanna's little games. Could she be in danger from her own child? And when it's her word against her daughter's, can she make her husband believe her?Bad Apple is a blazing debut novel about a perfect-looking family – where sweetness can be deceptive.Fans of BEHIND HER EYES, DAUGHTER or GOOD ME, BAD ME will love this page-turning story of dark domestic suspense."Unnerving and unputdownable, BABY TEETH will get under your skin and keep you trapped in its chilling grip until the shocking conclusion." New York Times bestselling author Lisa Scottoline"Gone Girl meets The Omen… a twisty delicious read" Entertainment Weekly*Bad Apple is also published in America under the title Baby Teeth.*
Bad Apples: Identify, Prevent & Manage Negative Behavior at Work
by A. Furnham J. TaylorIn a difficult economic climate it is more important than ever to manage bad behaviour in the workplace and minimize the damage negative and destructive employees can have on an organization. This book looks at the problems companies can face but also shows how to resolve these issues and work towards a positive outcome.