Advanced Photon Counting Applications, Methods, Instrumentation

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Synopsis

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Book details

Edition:
2015
Series:
Springer Series on Fluorescence (Book 15)
Author:
Peter Kapusta, Michael Wahl, Rainer Erdmann
ISBN:
9783319156361
Related ISBNs:
9783319156354
Publisher:
Springer International Publishing
Pages:
N/A
Reading age:
Not specified
Includes images:
Yes
Date of addition:
2019-09-07
Usage restrictions:
Copyright
Copyright date:
2015
Copyright by:
Springer International Publishing, Cham 
Adult content:
No
Language:
English
Categories:
Nonfiction, Science, Technology