Type 3 Secretion Systems Methods and Protocols

Synopsis

This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.

Book details

Edition:

1st ed. 2017

Series:

Methods in Molecular Biology (Book 1531)

Author:

Matthew L. Nilles and Danielle L. Jessen Condry

ISBN:

9781493966493

Related ISBNs:

9781493966479

Publisher:

Springer New York

Pages:

N/A

Reading age:

Not specified

Includes images:

Yes

Date of addition:

2021-01-31

Usage restrictions:

Copyright

Copyright date:

2017

Copyright by:

Springer New York, New York, NY 

Adult content:

No

Language:

English

Categories:

Nonfiction, Science