CRISPR-Cas Methods

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Synopsis

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

Book details

Edition:
1st ed. 2020
Series:
Springer Protocols Handbooks
Author:
M. Tofazzal Islam, Pankaj K. Bhowmik, Kutubuddin A. Molla
ISBN:
9781071606162
Related ISBNs:
9781071606155
Publisher:
Springer US
Pages:
N/A
Reading age:
Not specified
Includes images:
Yes
Date of addition:
2021-03-14
Usage restrictions:
Copyright
Copyright date:
2020
Copyright by:
Springer Science+Business Media, LLC 
Adult content:
No
Language:
English
Categories:
Nonfiction, Science