DNA Modification Detection Methods

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Synopsis

Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years.  This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.

Book details

Edition:
1st ed. 2022
Series:
Springer Protocols Handbooks
Author:
Bi-Feng Yuan
ISBN:
9781071612293
Related ISBNs:
9781071612286
Publisher:
Springer US
Pages:
N/A
Reading age:
Not specified
Includes images:
Yes
Date of addition:
2021-08-06
Usage restrictions:
Copyright
Copyright date:
2022
Copyright by:
Springer Science+Business Media, LLC, part of Springer Nature 
Adult content:
No
Language:
English
Categories:
Medicine, Nonfiction, Science, Technology