Genetically Incorporated Non-Canonical Amino Acids Methods and Protocols

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Synopsis

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.

Book details

Edition:
1st ed. 2023
Series:
Methods in Molecular Biology (Book 2676)
Author:
Yu-Hsuan Tsai, Simon J. Elsässer
ISBN:
9781071632512
Related ISBNs:
9781071632505
Publisher:
Springer US
Pages:
N/A
Reading age:
Not specified
Includes images:
Yes
Date of addition:
2023-07-06
Usage restrictions:
Copyright
Copyright date:
2023
Copyright by:
The Editor 
Adult content:
No
Language:
English
Categories:
Nonfiction, Science